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Types of Samples for DNA Testing & Test Success Rate

DNA testing can be performed using personal items or human tissue.

Samples other than cotton swabs for DNA testing

DNA testing is typically conducted by collecting buccal mucosa (cells from the inside of the cheek) using a specialized buccal swab.
However, testing can also be performed using other samples such as items touched by the subject, dandruff, nails, or body hair.

Please note that while seeDNA Genetic Medicine Laboratory clearly lists the success rates for each sample, many testing laboratories do not disclose their success rates due to high failure rates.

Rest assured that the accuracy of the test does not change depending on the type of sample used.

Success Rate of DNA Paternity Testing by Sample Type

The main reason a test success rate does not reach 100% is because the submitted sample contains DNA from multiple individuals, or the DNA within the sample has degraded.

In such cases, seeDNA will cover the return shipping costs and you can choose between a free retest using a buccal swab or a partial refund guarantee.

If even a trace amount of DNA remains in the sample, a clear result can almost certainly be obtained.

Except for liquids and blood, keeping all samples dry is crucial for preservation.
Since DNA is highly sensitive to ultraviolet light, please avoid storing samples in direct sunlight.
If stored for a long period, there is a risk of DNA degradation, so freezing is recommended.

Success rate of DNA paternity testing

seeDNA Genetic Medicine Laboratory guarantees accuracy that exceeds international standards, even for prenatal paternity DNA testing using various samples.

While buccal swab samples can be analyzed using a fully automated system, samples other than buccal swabs require manual processing by technicians with an average of 10+ years of experience, which requires additional days*1 and fees.

*1: If submitted before the swab or blood samples, testing will begin earlier, reducing the additional days required.

Additional Timeline & Fees (Tax Incl.)

Type
Collection Method
Buccal Mucosa (Swab Collection)
A buccal swab rubbed back and forth 10 times vertically and horizontally against the inside of the cheek
(Sterile swabs are included in the DNA testing kit we mail to you)
Additional Days : +0 days / Fee : 0 yen
Blood
At least 0.001 ml of blood collected and treated with EDTA
Additional Days : +2 days / Fee : 33,000 yen
Semen
Tissue paper, condom, cloth, etc., with semen attached (stored dry or frozen)
Additional Days : +2 days / Fee : 33,000 yen
Cigarette Butt
At least 1 cigarette butt (3 for prenatal DNA testing)
Storing in a dry state at room temperature is recommended
Additional Days : +2 days / Fee : 33,000 yen
Paper Cup
Using the area where the subject's mouth made contact
Storing in a dry state at room temperature is recommended
Additional Days : +2 days / Fee : 33,000 yen
Hair / Body Hair
At least 3 hairs or body hairs with roots attached (5 or more is recommended; naturally fallen hair is not accepted)
Additional Days : +2 days / Fee : 33,000 yen
Bloodstain
Tissue paper, condom, cloth, sanitary pad, etc. with bloodstains of 0.5 cm or more in diameter
Additional Days : +2 days / Fee : 33,000 yen
Toothbrush
Used at least once and dried naturally
Additional Days : +2 days / Fee : 33,000 yen
Nail
At least 5 pieces of fingernails/toenails cut with nail clippers, measuring 0.2 cm or more
Additional Days : +2 days / Fee : 33,000 yen
Umbilical Cord
Dry umbilical cord (0.5 cm or more in length is recommended)
※ We have successfully obtained clear results even from an umbilical cord stored at room temperature for 110 years
Additional Days : +2 days / Fee : 33,000 yen
Earwax
Earpick or cotton swab with at least 2 mg of earwax attached
Additional Days : +2 days / Fee : 33,000 yen
Chewing Gum
Collect sugarless or regular gum chewed for at least 5 minutes without touching it directly with bare hands
Additional Days : +2 days / Fee : 33,000 yen
Nasal Secretions (Mucus)
One sheet of tissue paper used to blow nose (stored dry)
Additional Days : +2 days / Fee : 33,000 yen
Chopsticks
Disposable chopsticks dried in the air after being used for a meal
Additional Days : +2 days / Fee : 33,000 yen
Shaver / Razor
The head portion of an electric shaver (can be tested even if washed with water)
Additional Days : +2 days / Fee : 33,000 yen
Bone (Uncremated)
Human bone that has not been cremated
Additional Days : +30 days / Fee : 100,000 yen
Bone (Cremated)
Cremated human bone
※ Only available for "Special DNA Testing"
Additional Days : +10 days / Fee : 33,000 yen
Tooth
Extracted tooth (teeth over 100 years old are acceptable)
Additional Days : +3 weeks / Fee : 50,000 yen (Consultation)
Diaper
Used diaper opened and completely dried
Additional Days : +2 days / Fee : 33,000 yen
Other
Tissue slices or sections, etc.
Additional Days : +2 days to 4 weeks / Fee : (Consultation)

※ If preserved under good storage conditions, DNA testing can be performed even on items over a million years old.

Storing samples in a wet state at room temperature or exposing them to direct sunlight (UV rays) increases the risk of not obtaining clear results.

If you are considering testing, please consult with our professional staff as early as possible.

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Toothbrush (Interdental Brush, Floss)

Toothbrush (Interdental Brush, Floss)

The most common and representative sample with the highest track record

Testing is possible if used at least once.
The shorter the storage period, the higher the test success rate. As long as it is washed with water and stored dry at room temperature, it can be tested without issues.

If stored for 3 months or longer, or stored wet at room temperature, the risk of DNA degradation increases.

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Cigarette (Paper Cigarette, E-Cigarette)

Cigarette (Paper Cigarette, E-Cigarette)

Success rate varies depending on smoking habits

Please provide at least 1 cigarette filter (3 for prenatal DNA testing) that has been smoked directly by mouth.
Please dry the filter thoroughly and avoid touching it directly with your hands.
Since everyone has different smoking habits, it may be difficult to collect DNA if the filter was touched by the mouth only briefly.

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Hair (Body Hair)

Hair (Body Hair)

The key point is the presence of the "root"

For DNA testing, the hair must have the hair follicle (root) attached. Please submit hair with roots.
Hair without roots can be used as a sample for maternal lineage testing or identity verification testing.

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Nail

Nail

It is recommended to provide at least 5 pieces of fingernails or toenails cut with nail clippers, measuring 0.2 cm or more.
As a guideline, please submit nails collected within the past month.

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Semen / Body Fluid

Semen / Body Fluid

Testing can be performed using tissue paper, condoms, underwear, etc., with semen or body fluid attached.

For semen collected in a condom, you can drop the semen onto a tissue, apply it to a testing swab, or tie the condom opening and return it.
If the semen has come into contact with a female body, multiple DNA profiles may mix, raising the risk of the test being inconclusive.

Sample Collection Methods

Samples required for DNA testing are called specimens.<br />Please check the video for correct collection and storage methods.

※ The video states "4 specialized swabs per subject" under "1. Buccal Swab Collection Method," but the correct quantity is "2 specialized swabs per subject."

An explanation video demonstrating buccal swab sample collection methods in 4 minutes.

  • 0:00~ What is a sample? (Specimen explanation)
  • 0:40~ Buccal swab sample collection method
  • 2:05~ Samples other than buccal swabs (Special specimens)
  • 2:38~ Example: Toothbrush storage & return method
  • 3:10~ Blood collection method (Prenatal DNA testing)

Desirable Sample Collection Cases

If even a trace*2 amount of DNA is present, test results can almost certainly be obtained.
Rest assured that testing is possible unless the sample is left wet at room temperature for a day or more, or is kept in extremely poor storage conditions.

*2: The amount of DNA required for a single test is one-billionth of a gram (1 ng).

Recommended Cases

  • The father candidates are in a sibling or parent-child relationship.
  • There are multiple father candidates.
  • Pregnancy was achieved through artificial insemination or IVF.
  • There were multiple partners around the time of conception.

※ If you have any questions, please feel free to consult with us.

Frequently Asked Questions

Frequently Asked Questions

Q.What types of samples can be used for DNA testing?

A.
In addition to standard buccal mucosa (swab), DNA testing can be performed using over 19 types of samples, including toothbrush, cigarette butt, hair (with root), nail, semen, blood, bloodstain, umbilical cord, earwax, chewing gum, nasal secretions, disposable chopsticks, paper cup, shaver, diaper, bone, and tooth. The testing accuracy does not change depending on the type of sample used(1).

Q.What are the additional fees and timelines when using samples other than buccal swabs?

A.
For specialized samples other than buccal swabs, manual DNA extraction is required, which generally adds 33,000 yen (tax included) and 2 business days. Uncremated bone adds 100,000 yen and 30 days, while teeth add 50,000 yen and 3 weeks, varying by sample type.

Q.What is the correct way to store samples?

A.
As a general rule, all samples except liquids and blood must be stored dry. Since DNA is sensitive to UV rays, please avoid direct sunlight. For long-term storage, freezing is recommended. Leaving a sample wet at room temperature for over a day increases the risk of DNA degradation, making testing impossible(2).

Q.Can hair be used for DNA testing?

A.
Yes, hair can be used if it has the root (follicle) attached. At least 3 hairs with roots (5 or more recommended) are required; naturally shed hair lacks sufficient root cells and cannot be used. Hair without roots can still be used for maternal lineage or identity verification testing.

Q.What happens if DNA cannot be detected from the sample?

A.
If DNA cannot be detected from the submitted sample, seeDNA will cover return shipping and offer a choice between a free retest using a buccal swab or a partial refund guarantee. The main reasons success rates do not reach 100% are the presence of DNA from multiple people or DNA degradation in the sample.